Lab Supplies

Add 200 μl of this buffer to tail. Boil for 30 min. Cool down. Use 1 μl DNA for genomic PCR
50 ml XpressLysis Buffer Cat# ATG-mDNA-50100 ml XpressLysis Buffer Cat# ATG-mDNA-100
This single buffer is to quickly lyse mouse tail/ear tissues to extract gDNA in just 30 minutes at boiling temperature, an inexpensive, convenient, and fast alternative protocol over the conventional Proteinase K based method. 1 μl gDNA containing solution can be directly used in a 20 μl PCR reaction for mouse genotyping.

Description:

A one step process to extract gDNA from mouse tail/ear tissues in 30 minutes with XpressLysis Buffer.

It is convenient: No further liquid transfer is necessary after adding 200 μl of this buffer into the tubes with tail (It is normal for residual tail tissue to remain in the liquid after the process).

It is fast: No long incubation time is required, taking only 30 minutes from start to finish.

It can be high throughput: Just use the PCR machine, set the temperature for 95°C for 30 min.

It is inexpensive: Compared to costly gDNA extraction kits, it helps save huge money and precious time.

HERE IS THE PROTOCOL.

To order: LARGE-test-tube iceman-rack, $208 per eachLARGE-test-tube iceman-rack holds most types of large-diameter test tubes that are used in daily biological and biomedical research, such as tissue collection & fixation, FACS, immunoprecipitation, seed cell banking, cell lysate preparation, protein & enzyme preparation, DNA & RNA preparation, and other assays at low temperature. This corrosion-proof rack is made of metal with thermal superconductivity and makes real-time changes (3-5 seconds) in temperature that fit your needs, ranging from -80ºC to +100ºC. It is an ideal benchtop cooling and storage device. The test tubes that fit into this rack are the ones with volumes of 2ml, 3ml, 4ml, 5ml, 6ml, 13 ml, and 15ml .

Color: black or gray
Size: 5.0 x 3.2 x 1.2 (inch)
Hole sizes in diameter: 16.3mm, 12.7mm, and 11.7mm, respectively
Material: metal with thermal ultraconductivity

To order:
SMALL-test-tube iceman-rack, $280 per each
MALL-test-tube rack holds most types of small-diameter test tubes that are used in daily biological research, such as DNA & RNA preparation, PCRs & RT-PCRs, molecular cloning, protein & enzyme preparation & purification, cell lysate preparation, immunoprepcipitation, tissue collection & fixation, and other assays at low temperature. The rack is made of metal with thermal superconductivity and makes realtime changes (3-5 seconds) in temperature that fits your needs, ranging from -80ºC to +100ºC and is corrosion-proof. The test tubes that fit into this rack are the ones with volumes of 0.6ml, 1.5ml, and 2.0ml.

Color: black or gray
Size: 6.2 x 4.2 x 1.2 (inch)
Hole sizes in diameter: 11mm and 8mm, respectively
Material: metal with thermal ultraconductivity

To order: 96-well plate iceman-rack, $280 per each96-well plate iceman-rack is specifically designated for facilitating large-scale PCR preparation. The rack holds a 96-well PCR plate, twelve 8-tube strips, or eight 12-tube strips that are used for preparing PCR arrays, genotyping transgenic and knockout mice, screening clones or colonies, running PCR & RT-PCR automation, and benchtop cooling & storage, etc. The rack is made of metal with thermal superconductivity and makes realtime changes (3-5 seconds) in temperature that fits your needs, ranging from -80ºC to +100ºC and is corrosion-proof. The plate or tube strips that fit into this rack are the ones with volumes of 0.2ml per well/tube.

Color: black or silver
Size: 4.6 x 3 x 0.6 (inch)
Material: metal with thermal ultraconductivity
Fitting PCR Plates: standard PCR plates, including that from Applied Biosystems, ABgene, and Bio-Rad; ABgene SuperPlate, and Bio-Rad Hard-Shell

To order,
384-Well-PCR-Plate Rack Catalog # RKP384: $310

384-well plate rack is specifically designated for facilitating large-scale PCR preparation. The rack holds a 384-well PCR plate that are used for preparing PCR arrays, screening clones or colonies, running PCR & RT-PCR automation, and benchtop cooling & storage, etc. The rack is made of metal with thermal superconductivity and makes realtime changes (3-5 seconds) in temperature that fits your needs, ranging from -80ºC to +100ºC and is corrosion-proof.

Color: black or silver
Size: 4.6 x 3 x 0.6 (inch)
Material: metal with thermal ultraconductivity
Fitting PCR Plates: standard PCR plates, including that from Applied Biosystems, Roche, Corning, Eppendorf, Greiner, Nunc, and other suppliers.

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