Antibody-dependent cellular cytotoxicity (ADCC) assay, a cell -based assay, can be performed by us upon request from customers. For your custom ADCC bioassay, we will provide our ADCC platform, including Jurkat cell line with specific Fc receptors, custom antigen presenting cell lines and custom antibody. For inquiry, please contact us
Background
ADCC is an immune defense mechanism involving an effector cell lysing a target cell on which antibodies have bound to specific antigens on the target cell membrane.
The classical ADCC bioassay measures the lysis of target cells by NK cells, using release of labeled radioactivities (51Cr) or enzymes (such as LDH) as readout. Nowadays, a cell-based assay measuring the activation of effector cells (Jurkat) upon Fc receptors engagement and crosslinking by target cells that are amplified by luciferase signals is more routinely adopted to replace the classical method.
Reference:
Cross-species higher sensitivities of FcγRIIIA/FcγRIV to afucosylated IgG for enhanced ADCC. Mao C, Near R, Zhong X, Gao W. Antib Ther. 2021 Aug 19;4(3):159-170. doi: 10.1093/abt/tbab016. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8408537/
List of available Jurkat cell lines with added FcγR for ADCC assay:
For information, click Jurkat cell lines below, To purchase, click Purchase below.
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